FIG. 1.

Analysis of direct CTL activity and CTLp levels, T-cell counts, and viral load measurements from the SBBC members with detectable plasma viremia: D36, C18, C54, and C98. (A to D) Responses to env, gag, pol, and nef determinants are shown. Peptide determinants in panels A and B are listed in Table 2. Direct CTL activity was measured by a peptide-induced ELISPOT assay (IFN-γ-releasing cells/10⁶ PBMC) (A), a tetramer-binding assay (percent CD8⁺ T cells) (B), and direct lysis of recombinant vaccinia virus-infected BLCL (effector/target cell ratio, 50:1) (C). CTLp levels were measured against recombinant vaccinia virus-infected BLCL (D). (E and F) CD4 and CD8 counts (cells per microliter) (E) and plasma RNA viral load measurements (PCR) (F) determined during the study period. ND, tests not done.