Table 1.
Loci | PCR Primers | Sequence (5′—3′) | PCR Cycles | References |
---|---|---|---|---|
ITS |
ITS5 ITS4 |
GGA AGT AAA AGT CGT AAC AAG G TCC TCC GCT TAT TGA TAT GC |
(95 °C: 30 s, 55 °C: 30 s, 72 °C: 1 min) × 35 cycles | [23] |
tef1α |
EF1-728F EF-2 |
CAT CGA GAA GTT CGA GAA GG GGA RGT ACC AGT SAT CAT GTT |
(95 °C: 30 s, 48 °C: 30 s, 72 °C: 1 min) × 35 cycles | [24,25] |
tub2 |
Bt-2a Bt-2b |
GGT AAC CAA ATC GGT GCT GCT TTC ACC CTC AGT GTA GTG ACC CTT GGC |
(95 °C: 30 s, 53 °C: 30 s, 72 °C: 1 min) × 35 cycles | [26] |