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. 2023 Jun 21;11(7):1627. doi: 10.3390/microorganisms11071627

Table 1.

Molecular markers and their PCR primers and programs used in this study.

Loci PCR Primers Sequence (5′—3′) PCR Cycles References
ITS ITS5
ITS4
GGA AGT AAA AGT CGT AAC AAG G
TCC TCC GCT TAT TGA TAT GC
(95 °C: 30 s, 55 °C: 30 s, 72 °C: 1 min) × 35 cycles [23]
tef1α EF1-728F
EF-2
CAT CGA GAA GTT CGA GAA GG
GGA RGT ACC AGT SAT CAT GTT
(95 °C: 30 s, 48 °C: 30 s, 72 °C: 1 min) × 35 cycles [24,25]
tub2 Bt-2a
Bt-2b
GGT AAC CAA ATC GGT GCT GCT TTC
ACC CTC AGT GTA GTG ACC CTT GGC
(95 °C: 30 s, 53 °C: 30 s, 72 °C: 1 min) × 35 cycles [26]