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Gate strategy to set T lymphocytes by flow cytometry. (A) Gate strategy to set T (CD3+) from peripheral blood mononuclear cells. (B) Subsets of TCD4+/TCD8+ according CD3+ and CD4+ or CD8+ expression (C) Gating of activated TCD4+/TCD8+ (Ox40+ CD38+); (D) Percentage of TEMRA (CD45RA+CCR7-), naïve (CD45RA+CCR7+), EM (CD45RA-CCR7-) and CM (CD45RA-CCR7+) TCD4+/TCD8+.
