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Southern blot analysis of nucleic acid from cells expressing Ty3 IN mutants. A 10-μg sample of total nucleic acid digested with BamHI was separated on a 1% agarose gel, transferred to nitrocellulose (Duralon UV; Stratagene) and probed with a 32P-labeled, BglII-digested DNA fragment containing the internal (non-LTR) region of Ty3. The position of the 5.4-kb replicated, full-length, extrachromosomal linear Ty3 DNA is indicated; the upper band represents the expression plasmid (P).
