Table 1.

Gene sequences of the PCR primers.

Gene	Accession Number		Sequence (5′-3′)	Product Size (bp)
Bacs	NM_009512.2	F	TCT ATG GCC TAA AGT TCA GGC G	75
		R	CTT GCC GCT CTA AAG CAT CC
Baat	NM_007519	F	GTG TAG AGT TTC TCC TGA GAC AT	199
		R	CTG GGT ACA GGT GGG TAG AC
Bsep	NM_021022	F	AGC AGG CTC AGC TGC ATG AC	122
		R	AAT GGC CCG AGC AAT AGC AA
Cdo	NM_033037.4	F	GGG GAC GAA GTC AAC GTG G	162
		R	ACC CCA GCA CAG AAT CAT CAG
Csd	NM_001359126	F	CCA GGA CGT GTT TGG GAT TGT	193
		R	ACC AGT CTT GAC ACT GTA GTG A
Cyp7a1	NM_007824	F	AAG AGC AAC TAA ACA ACC TG	244
		R	TTC CCA CTT TCA TCA AGG TA
Fmo1	NM_010231.3	F	CCA TCA AGT GCT GCC TGG AA	143
		R	CCT GCT GCT GTT AGA AAC CAC AGA
Fmo3	NM_008030.1	F	CCA CAG CAG GGA CTA TAA GGA A	129
		R	GAG CTG ATG GTG ACC TTC TGA
Fxr	NM_001163700	F	GGT CAT GCA GAC CTG TTG GAA	142
		R	TGA CGA TCG CTG TGA GCA GA
Hnf4α	NM_008261.3	F	ATG CCT GCC TCA AAG CCA TC	67
		R	ATC TTG CCC GGG TCA CTC A
Ntcp	NM_001177561	F	AAG GCC ACA CTA TGT ACC CTA CGT C	106
		R	GAT GCT GTT GCC CAC ATT GA
Shp	NM_011850	F	CAA GGA GTA TGC GTA CCT GA	232
		R	GAT AGG GCG GAA GAA GAG AT

Bacs, ATP-dependent microsomal bile acid CoA synthetase; Baat, bile acid-CoA:amino acid N-acetyltransferase; Bsep, bile salt export pump; Cdo, cysteine dioxygenase; Csd, cysteine sulfinate decarboxylase; Cyp7a1, cytochrome P450 7a1; Fmo1/3, flavin containing monooxygenase 1/3; Fxr, farnesoid X receptor; Hnf4α, hepatocyte nuclear factor 4α; Ntcp, sodium/taurocholate co-transporter peptide; Shp, small heterodimer partner; F, forward; R, reverse; bp, base pair.