FIG. 9.

Analysis of reactivation and recurrent infection in the lungs. Recurrence of infectious with virus from latently infected lungs was induced at 12 months after BMT and infection by total-body γ-irradiation with a dose of 6.5 Gy. Data are shown for a particular case on day 14 after the irradiation. (A) Assay of infectivity. Infectious virus was detected in lung tissue pieces #10 through #18 (left lungs and postcaval lobe) by using the RT PCR-based focus expansion assay (details are as described in the legend for Fig. 3). (B) Detection of IE1 and IE3 transcripts. Poly(A)⁺ RNA was isolated from lung tissue pieces #1 through #9 (remaining three lobes) on day 14 after the irradiation, and 1/10 aliquots (ca. 200 ng) were subjected to the RT PCRs.