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. 2023 Jul 21;16(7):1034. doi: 10.3390/ph16071034

Figure 2.

Figure 2

Amlexanox restores GDAP1 mRNA expression in NPs carrying nonsense mutation (c.581C>G, p.Ser194*). The NPs of patient were treated with amlexanox (100 µM), or G418 (1 mg/mL), G418 was used as positive control. After 20 h of treatment, total RNAs were extracted. Reverse transcription and PCR was performed to measure the level of GDAP1 mRNA. To normalize the amount of PTC-containing mRNA, TBP was used as the reference gene. NTT: non treated cells; AML: amlexanox. The nonparametric Mann–Whitney test was performed; * p < 0.05. n = 3.