FIG. 1.

Efficiency of 208F cell transduction following exposure to the LAPSN(PA317) vector. 208F cells were seeded at 5 × 10⁴ cells per 6-cm dish and exposed to LAPSN(PA317) vector beginning the day after seeding. Flow cytometric analysis of alkaline phosphatase expression was performed 2 days after the last exposure to vector. (A) Percentage transduction after a single exposure to various amounts of vector. (B) Percentage transduction after one to six daily exposures to a constant volume of 1 ml of vector. Values represent means ± standard deviations of three independent experiments.