Figure 3.
(A). Effect of NG in co-exposure with 130 mM glucose-induced intracellular ROS in the ocular region of the zebrafish larvae using DCFDA fluorescent probe. The retina has been highlighted in the white box. The fluorescence image was captured using a fluorescence microscope. (C) Experiments were performed in triplicate, and the data were expressed as mean ± SD. *** represents the statistical significance at p < 0.001 compared to the control. (B). Effect of NG in co-exposure with 130 mM glucose-induced intracellular O−2 in the ocular region of zebrafish larvae using NDA fluorescent probe. The ocular region has been highlighted in the white box. The fluorescence image was captured using a fluorescence microscope. (D) Experiments were performed in triplicate, and the data were expressed as mean ± SD. ** represents the significant difference p < 0.01 and *** represents p < 0.001. (C,D) Fluorescent intensity of the zebrafish larvae calculated using Image J software.