Seroprevalence of anti-Borrelia IgG in tick-infested patients and healthy donors according to gender, settlement, and age. (a) Comparison of total prevalence values between the studied groups. (b) Comparison between the groups of males and also between females from tick-infested patients and healthy donors living in Skopje and Novi Sad. (c) The tick-infested patients and donors were compared according to location of residence (Skopje vs. Novi Sad). (d) Comparisons were performed in each age group as well, despite no children or senior individuals being present in the cohort of healthy donors from Novi Sad and Skopje. RR: relative risk, indicates the probability of an individual in the groups of “tick-infested patients” to be seropositive to Borrelia. RR = ∞ (undefined) when the prevalence value equals 0 in a group. The significance of the association was also tested using Fisher’s exact test (* p < 0.05; ns, non-significant).