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. 2023 Jul 29;42:188. doi: 10.1186/s13046-023-02766-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — The cellular uptake and cytotoxicity of HTS NPs in vitro. (A-C) Confocal fluorescence images displaying cellular uptake of HTS NPs. Scale bar: 100 μm. (D-E) Flow cytometry analysis of the cellular uptake of HTS NPs. (G-I) Confocal fluorescence images and flow cytometry analysis showing cellular uptake of free Ce6, T-Ce6 NPs and HT-Ce6 NPs. Scale bar: 100 μm. (J-L) Confocal fluorescence images and flow cytometry analysis showing cellular uptake of HT-Ce6 NPs with or without HA. Scale bar: 100 μm. (M) The viability of 16HBE, H460 and A549 cells following treatment with HTS NPs. The error bars represent the means ± SD (n = 3)