Table 1.
Gene | Forward Primers (5′–3′) | Reverse Primers (5′–3′) |
---|---|---|
COL2 | GCAGCTGTGTGCAGGAGGGGAAG | TGGCAGTGGCGAGGTCAGTAGGG |
ACAN | GACTCATTGTTAGAGGACAGCCA | CACTCCCAAAAAGAACTCCAGAT |
PRG4 | GGCAGGGAATGTGACTGTGATG | TGGGTGAGCGTTTAGTTGTTGA |
SOX9 | CGGCGGAGGAAGTCGGTGAAGA | AGTGGTGGGTGGGGTGGTGGTG |
GAPDH | CATCAAGAAGGTGGTGAAGCAGG | AGCATCGAAGGTAGAGGAGTGGG |
COL2: type II collagen; ACAN: aggrecan; PRG4: proteoglycan 4 precursors; SOX9: SRY-related high mobility group-box gene 9; and GAPDH: glyceraldehyde-3-phosphate dehydrogenase.