IAT Method	Antiglobulin	CAPIL			GEL LAB			GEL KIT	STRIP KIT	MICRO
	Temperature	IgG 22 °C	RAD 22 °C	GAD 22 °C	IgG 22 °C	RAD 22 °C	GAD 22 °C	RAD 22 °C	RAD 22 °C	IgG 4 °C	IgG 37 °C	IgG 22 °C	RAD 4 °C	RAD 37 °C	RAD 22 °C	GAD 4 °C	GAD 37 °C
MICRO	GAD 22 °C	0.61	0.66	0.42	0.45	0.62	0.59	0.55	0.42	0.68	0.68	0.68	0.57	0.51	0.70	0.71	0.84
	GAD 37 °C	0.62	0.59	0.54	0.44	0.75	0.59	0.43	0.38	0.53	0.53	0.53	0.45	0.39	0.56	0.72
	GAD 4 °C	0.39	0.56	0.46	0.26	0.59	0.53	0.34	0.28	0.41	0.41	0.41	0.36	0.31	0.45
	RAD 22 °C	0.68	0.65	0.34	0.58	0.56	0.45	0.71	0.34	0.86	0.86	0.86	0.85	0.77
	RAD 37 °C	0.61	0.65	0.23	0.47	0.39	0.31	0.84	0.23	0.71	0.71	0.71	0.92
	RAD 4 °C	0.56	0.60	0.26	0.53	0.45	0.36	0.86	0.26	0.79	0.79	0.79
	IgG 22 °C	0.67	0.71	0.38	0.63	0.61	0.50	0.76	0.38	1.0	1.0
	IgG 37 °C	0.67	0.71	0.38	0.63	0.61	0.50	0.76	0.38	1.0
	IgG 4 °C	0.67	0.71	0.38	0.63	0.61	0.50	0.76	0.38
STRIP KIT	RAD 22 °C	0.23	0.11	0.15	0.19	0.23	0.28	0.25
GEL KIT	RAD 22 °C	0.60	0.63	0.25	0.51	0.43	0.34
GEL LAB	GAD 22 °C	0.39	0.46	0.64	0.63	0.72
	RAD 22 °C	0.62	0.68	0.69	0.66
	IgG 22 °C	0.55	0.44	0.46
CAPIL	GAD 22 °C	0.45	0.43
	RAD 22 °C	0.80
IAT methods: STRIP KIT: in-clinic immunochromatographic strip kit; CAPIL: microcapillary tube; MICRO: microtiter plate; GEL KIT: in-clinic gel minitube kit; GEL LAB: laboratory neutral gel column card. Antiglobulin reagents: GAD: goat anti-dog IgG, IgM, and C3; RAD: rabbit anti-dog IgG, IgM, and C3; IgG: rabbit anti-dog IgG. Groups were compared by Cohen’s kappa (κ). Interpretation of the κ value as per Landis and Koch [28], adapted by Brennan and Silman [29]: Green: very good agreement (≥0.81); orange: good agreement (≥0.61); blue: moderate agreement (≥0.41); purple: fair agreement (≥0.21), red: poor agreement (≤0.20). Interpretation of the κ value, as per Landis and Koch [28] adapted by McHugh [30]: strong agreement (≥0.8); moderate agreement (≥0.6); weak agreement (≥0.4); minimal agreement (≥0.21), no agreement (≤0.2).