Skip to main content
. 1999 Jan;73(1):576–584. doi: 10.1128/jvi.73.1.576-584.1999

FIG. 2.

FIG. 2

Inducible envelope and packaging constructs. pBIGFVG is an inducible plasmid that expresses the VSV-G envelope and the GFP genes under the control of a bidirectional tetracycline-inducible promoter. This promoter contains two human CMV minimal promoters (ph1 and ph2) separated by the TRE. Arrows indicate transcription directions. The simian virus 40 (SV40) and β-globin polyadenylation signals are indicated. The pPTK plasmid contains an HIV-1 genomic fragment that encodes all HIV-1 genes excluding the HIV-1 envelope (ΔR.8.2) under the regulation of an inducible promoter. This promoter contains the TRE and the human CMV minimal promoter (pHCMV). Also indicated are the bovine growth hormone polyadenylation signal (BGHpA), the SV40 promoter and origin (SV40ori), the neomycin resistance gene (Neo), the SV40 polyadenylation signal (SV40pA), the ColE1 origin (ColE1 ori), and the f1 origin (f1ori).