TABLE 3.
In vitro transduction of nondividing cellsa
| Cell type | Vector titer (IU/ml) under conditions of:
|
|
|---|---|---|
| Cycling | G1/S arrest | |
| HEF | 5 × 105 | 4 × 105 |
| HeLa | 1 × 106 | 8 × 105 |
a
Lentivirus vectors generated by the SODk1CG1 producer cell line were titrated by serial dilutions on HEF and HeLa cells arrested in G1/S by serum starvation for 48 h or by aphidicolin treatment, respectively. Vector titration on nontreated HEF and HeLa cells served as a control. The titers are averages of results from two independent experiments; there was no more than 25% variation between the experiments.