Fig. 5.

Effect of JQ1 on TNFα stimulated IL-6 and CXCL8 levels in HPASMC. Control (lefthand panels) and PAH (righthand panels) HPASMCs were pre-incubated with JQ1 + or JQ1- for 2 h prior to the addition of TNFα (10 ng/ml) for 24 h. Culture media was collected and levels of IL-6 A and B and CXCL8 C and D determined by ELISA. Data are shown as mean ± SEM of N = 8 experiments (4 Donors at 2 different passages). Statistical comparisons were made using Kruskal-Wallis one-way ANOVA with Dunns post-test. *p < 0.05, **p < 0.01, ***p < 0.001 when compared to Media control and ^#p < 0.05, ^##p < 0.01 when compared to TNFα