Figure 3.

The effectiveness of glutaraldehyde in inactivating SARS-CoV-2. (a) SARS-CoV-2 infectivity of samples incubated with 0.004% glutaraldehyde, for 0, 24, and 48 h, as determined by TCID₅₀ assays. Inactivated samples represented by orange bars, non-inactivated virus control (C+) represented by blue bars, and negative control (C-, inactivating agent) represented by the white bar. (b) RT-qPCR quantification of molecular targets N1 (white bars) and N2 (blue bars) in glutaraldehyde-inactivated samples and (c) samples from 5 serial passages of SARS-CoV-2 incubated with 0.004% glutaraldehyde for 24 h. (d) Representative TEM image of SARS-CoV-2 incubated with 0.004% glutaraldehyde for 24 h. Scale bar, 100 nm; SP, serial passage, C+, positive control; C-, negative control.