Table 1.

Experimental Schedule for the Chronic Unpredictable Stress Procedure

W/D	Day 1	Day 2	Day 3	Day 4	Day 5	Day 6	Day 7
Week 1	Noise 1hr	Food deprivation (6h during dark)	Inversion of light and dark cycle	5 min cold water swim	4h Stroboscope	Restrain	4h wet bedding
Week 2	Food deprivation (6h during dark)	Inversion of light and dark cycle	5 min cold water swim	4 h Stroboscope	Restrain	4h wet bedding	Noise 1hr
Week 3	Inversion of light and dark cycle	5 min cold water swim	4h Stroboscope	Restrain	4h wet bedding	Noise 1hr	Food deprivation (6h during dark)
Week 4	5 min cold water swim	Restrain	4H wet bedding	4 h Stroboscope	Food deprivation (6h during dark)	Noise 1 hr	Inversion of light and dark cycle
Week 5	OFT, EPM, Restrain	MWM, Food deprivation (6hr during dark)	MWM, 4 hStroboscope	MWM, 4H wet bedding	MWM, Noise 1hr	MWM (Probe trail), 5 min cold water swim	FST, blood collection

Abbreviations: MWM, Morris water test; OFT, open field test; EPM, elevated plus maze test; FST, forced swim test.