Figure 6.

Phagocytosis of Sporothrix schenckii, Sporothrix brasiliensis, and Sporothrix globosa by human monocyte-derived dendritic cells. Fungal cells were labeled with Acridine Orange and incubated with human monocyte-derived dendritic cells at an immune cell-fungus ratio of 1:6, for 2 h at 37°C and 5% (v/v) CO₂. Human cells were analyzed by flow cytometry, collecting 50,000 events, which were defined as a human cell interacting with at least one fluorescent fungal cell. Control, human cells were incubated only with PBS. *P < 0.05 when compared to conidia from the same species. ^†P < 0.05 when compared with the same morphology in the other two species. Results are shown as mean ± standard deviation from data generated with samples from eight donors analyzed by duplicate.