Persistent virus replication in HepG2 cells. Cultures of HepG2 cells (105) were infected with V8A, V10B, V30B, V51A, V51B, MHV-A59, or the MHV-H2 host range variant at an MOI of 5 for 1 h at room temperature. Virus samples were harvested at the designated times and assayed by plaque assay.