FIG. 3.

Blockade experiments in HepG2 cells. Cultures of HepG2 cells in eight-chamber LabTek slides were untreated (w/o) or treated with various polyclonal or monoclonal antibodies against hCEA genes at a 1:4 dilution for 1 h at room temperature. The antibodies were removed, and the cultures were infected with V51B at an MOI of 5 for 1 h. The virus inoculum was removed, and complete medium containing a 1:20 dilution of the same antibody was added to the chamber. Virus samples were taken at the indicated times, and virus titers were determined by plaque assay in DBT-9 cells.