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pSinRep19 replicons do not inhibit MHV infection. To determine if the noncytopathic pSinRep19 replicons block MHV replication, cultures of BHK-MHVR cells were transfected with the pSinRep19/T7pol transcripts and selected with puromycin (5 μg/ml) for several days. Cultures of BHK-MHVR/SinT7pol, BHK-MHVR, and BHK cells were infected with MHV-A59 at an MOI of 5 for 1 h. Virus samples were harvested at the indicated times and assayed by plaque assay in DBT cells.
