MHV replication in BHK cells expressing hCEA glycoproteins. Cultures of BHK, BHK-hCEA, BHK-hBgp1, and BHK-hBgp1+ cells were infected with V51B or MHV-A59, as indicated, at an MOI of 5 for 1 h at room temperature. In some experiments, the cells had been pretreated with a 1:4 dilution of pCEA or nonspecific antibody EDDA for 30 min prior to infection. Following infection, the cultures were washed extensively and virus were samples taken at the indicated times.