Table 1.

Monoclonal anti-fentanyl antibodies cloned from BCRs of B cells (MBC ID) belonging to the switched memory B cell population

Mouse	MBC ID	Heavy V chain	Heavy D chain	Heavy J chain	Heavy CDR3 aa	Heavy constant	Heavy SHM	Light V chain	Light J chain	Light CDR3 aa	Light constant	Light SHM
1	196	IGHV1–53	IGHD1–1	IGHJ2	AIEVGYYDY	Ighg2b	6	IGKV6–15	IGKJ2	EQYNS YPYT	Igkc	3
1	208	IGHV1–53	IGHD1–1	IGHJ2	AIEVGYYDY	Ighg2b	6	IGKV6–15	IGKJ2	EQYNS YPYT	Igkc	3
1	24	IGHV1–74	IGHD2–3	IGHJ4	AIEIYDGYY AMDY	Ighg1	2	IGKV6–15	IGKJ5	QQYNS YPLT	Igkc	1
1	136	IGHV1–74	IGHD2–3	IGHJ4	AIEIYDGYN TMDY	Ighg1	2	IGKV6–15	IGKJ5	QQYNN YPLT	Igkc	1
1	156	IGHV1–75	IGHD1–1	IGHJ2 YFDY	ARRDYGSS	Ighg1	0	IGKV1–99	IGKJ4	FQSN YLPLT	Igkc	0
6	609	IGHV1–74	IGHD1–1	IGHJ2	AMEDYYGS SYEDY	Ighg1	3	IGKV6–15	IGKJ2	QQYNT YPYT	Igkc	1
6	949	IGHV1–74	IGHD1–1	IGHJ2	AIERDYYGS REDY	Ighg1	0	IGKV6–15	IGKJ2	QQYNS YPYT	Igkc	2
6	913	IGHV1–85	IGHD1–1	IGHJ1	AIEGFTTVV ARNFDV	Ighg1	4	IGKV6–15	IGKJ2	QQYNT YPYT	Igkc	3
6	861	IGHV2–2	IGHD1–3	IGHJ2	ATEVGYFDY	Ighg2b	2	IGKV6–15	IGKJ2	QQYNS YPYT	Igkc	1

The gene usage of the V(D)J combination is indicated, along with the CDR3 amino acid sequence, and the constant region for each heavy and light chain combination. In the “SHM” column, the number of silent and missense mutations detected along the full-length of the heavy or light chain is indicated.