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Effects of JDV Tat on different viral promoters in CV-1 and FBL cells
| Promoter constructa | Fold activationb in:
|
|
|---|---|---|
| CV-1 cells | FBL cells | |
| pJDV-U3R | 22.0 ± 2.3 | 8.0 ± 2.7 |
| pHIV2-LTR-CAT | 16.7 ± 4.9 | 9.4 ± 0.6 |
| pSIV-LTR-CAT | 20.5 ± 7.8 | 5.4 ± 0.7 |
| pHTLV-LTR-CAT | 3.2 ± 1.1 | 1.8 ± 0.4 |
| pRSV-LTR-CAT | 5.1 ± 1.2 | 2.9 ± 0.3 |
For each transfection, 0.5 μg of promoter plasmid and 1 μg of JDV tat plasmid were used.
Fold activation was calculated as described in the legend to Fig. 2.
