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. 1999 Jan;73(1):667–675. doi: 10.1128/jvi.73.1.667-675.1999

FIG. 6.

FIG. 6

Results of bootscanning of the S segments of Kosice strains and schematic representation of TUL N-protein sequence variation. (a) Bootscan searches of 300-nt S-segment regions with 150-nt overlap, using the FM algorithm. Bootstrap probabilities of joining Kosice strains with the Malacky/Moravia lineage, the Tula lineage, or the outgroup (ILV) are represented by a solid line, dots, and a dashed line, respectively. The 70% bootstrap cutoff level (15, 52) is indicated by a thin dashed line. (b) Amino acid sequence variation of N proteins of all TUL strains. Sites where TUL strains differ in the N-protein sequence are indicated under the black bar, representing the N-protein-encoding sequence in the center of the figure; vertical numbers denote codon positions. Amino acid residues identical in Kosice and other TUL strains are boxed; horizontally hatched rectangles mark residues identical in the N proteins of Malacky/Moravia and Kosice strains, vertically hatched rectangles indicate residues identical in N proteins of Tula and Kosice strains. (c) Locations of Malacky/Moravia-like (horizontally hatched) and Tula-like (vertically hatched) parts in the TUL/Kosice S segment. Recombination points identified by bootscanning (above 70% FM values) are indicated by black arrows; the recombination point deduced from aa pattern is indicated by a white arrow.