30% probe hybridization buffer	For 40 mL of solution
30% formamide	12 mL formamide
5× sodium chloride sodium citrate (SSC)	10 mL of 20× SSC
9 mM citric acid (pH 6.0)	360 μL 1 M citric acid, pH 6.0
0.1% Tween 20	400 μL of 10% Tween 20
50 μg/mL heparin	200 μL of 10 mg/mL heparin
1× Denhardt’s solution	800 mL of 50× Denhardt’s solution
10% dextran sulfate	8 mL of 50% dextran sulfate