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Analysis of UL69 expression in HF cells from a recombinant retrovirus by Western blotting with a UL69-specific monoclonal antibody. Samples are from cells that were infected with retro-UL69 at a multiplicity of 5 fluorescence units per cell and harvested at 0 h (lane 1), 12 h (lane 2), 24 h (lane 3) and 48 h (lane 4) after infection. The broad smear includes bands corresponding to the multiple UL69 phosphoforms.
