KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-Ly6G clone 1A8 | BioXCell | BE0075-1 |
| isotype control clone 2A3 | BioXCell | BE0089 |
| anti-CD45-AF700 | BioLegend | 103127 |
| anti-CD11b-AF594 | BioLegend | 101254 |
| anti-CD11c-Bv605 | BioLegend | 117334 |
| anti-SiglecF-APC-Cy7 | BD Biosciences | 565527 |
| anti-Epcam-FITC | BioLegend | 118207 |
| anti-F4/80-Pe-Cy7 | BioLegend | 123114 |
| anti-Ly6C-Bv421 | BioLegend | 128032 |
| anti-Ly6G-PerCp-Cy5.5 | BioLegend | 127616 |
| Bacterial and virus strains | ||
| USA300 LAC | Our laboratory | N/A |
| USA300 LAC-YFP | This study | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Luria Bertani Broth | Becton Dickinson | 244610 |
| Agar | Sigma | 400400010 |
| Chloramphenicol | Sigma | C-0378 |
| eBioscience RBC Lysis Buffer | Invitrogen | 00-4333-57 |
| autoMACS Rinsing Solution | MACS Miltenyi | 130-091-222 |
| Bovine Serum Albumin | MACS Miltenyi | 130-091-376 |
| RPMI 1640 with L-glutamine | Corning | 10-040-CV |
| Fetal Bovine Serum | Sigma | F4135 |
| Itaconate | Aldrich | 129204 |
| Sodium Hydroxide | Fisher Scientific | SS-2661 |
| LIVE/DEAD Stain | Invitrogen | L23105A |
| Paraformaldehyde | Electron Microscopy Sciences | 15714-S |
| Collagenase I | Gibco | 17100-017 |
| Dispase | Gibco | 17105-041 |
| Elastase | Worthington | LS006365 |
| DNAse | Invitrogen | 2224G |
| PowerUp SYBR Green PCR Mastermix | Applied Biosystems | A25742 |
| Methanol | Alpha Aesar | 22909 |
| XF Base Medium | Agilent | 102353-100 |
| Glutamine | LifeLine | LS-1031 |
| HBSS | Corning | 21-023-CV |
| Ferrocytochrome C from Bovine Heart | Millipore Sigma | C3131 |
| Diphenyleneiodonium Chloride | Millipore-Sigma | D2926 |
| Phorbol Myristate Acetate | Sigma-Aldrich | P8139 |
| NP-40 | Thermo Scientific | 28324 |
| Cytochalasin D | Sigma-Aldrich | C8273 |
| Lysostaphin | Sigma-Aldrich | L7386 |
| Formalin-Free tissue Fixative | Sigma | A5472 |
| Critical commercial assays | ||
| MACS Miltenyi Mouse Neutrophil Isolation kit | MACS Miltenyi | 130-097-658 |
| Mouse Cytokine Proinflammatory Focused 10-plex Discovery Assay | Eve Technologies | MDF10 |
| Mouse Cytokine/Chemokine 31-plex Discovery Assay | Eve Technologies | MD31 |
| E.Z.N.A. Total RNA Kit | Omega Bio-tek | R6834-02 |
| DNA-free DNA removal kit | Fisher Scientific | AM1906 |
| High Capacity cDNA Reverse transcription kit | Applied Biosystems | 43688-14 |
| LDH Release Assay | Abcam | ab65393 |
| Albumin (BCG) Assay Kit (Colorimetric) | Abcam | ab235628 |
| Deposited data | ||
| Raw and Analyzed scRNA-Seq Data | This study | GEO: GSE215195 |
| Experimental models: Organisms/strains | ||
| Irg1−/− C57BL/6NJ mice | Jackson Laboratories | 029340 |
| WT C57BL/6NJ mice | Jackson Laboratories | 005304 |
| Oligonucleotides | ||
| Mouse Irg1 F: CGTGAGAAAGCACCTTGTGA |
This study | N/A |
| Mouse Irg1 R: CTGTGGAAGGATGGGACAGT |
This study | N/A |
| Mouse actin F: AGCCATGTACGTAGCCATCC |
This study | N/A |
| Mouse actin R: CTCTCAGCTGTGGTGGTGAA |
This study | N/A |
| Software and algorithms | ||
| FlowJo v10 | FlowJo | https://www.flowjo.com/ |
| FACSDiva v9 | BD Biosciences | https://www.bdbiosciences.com/en-us/products/software/instrument-software/bd-facsdiva-software |
| Cell Ranger | 10X Genomics | https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger |
| Seurat v4.0.4 | Satija Laboratory | https://satijalab.org/seurat/ |
| R v4.1.1 | R Project | https://www.r-project.org/ |
| StepOne v2.3 | ThermoFisher | https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/StepOne-and-StepOnePlus-Real-Time-PCR-System.html |
| E-Maven v0.10.0 | Rabinowitz Laboratory | http://maven.princeton.edu/index.php |
| Seahorse Wave Desktop v2.6.0 | Agilent | https://www.agilent.com/en/product/cell-analysis/real-time-cell-metabolic-analysis/xf-software/seahorse-wave-desktop-software-740897 |
| Prism v9.2.0 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| Other | ||
| LS Column | MACS Miltenyi | 130-042-401 |
| MidiMACS Separator magnet | MACS Miltenyi | 130-042-302 |
| 40 μm cell strainers | Falcon | 352340 |
| 70 μm cell strainers | CELLTREAT | 229483 |
| XFe24 sensor cartridge | Agilent | 102340-100 |
| XF24 well plate | Agilent | 102340-100 |