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Increased HLA-E expression on 5GKO.HLA-E cells after pulsing with HLA-G VL9 peptides. 5GKO.HLA-E cells were incubated with HLA-G VL9 peptides at 25 µM, 50 µM, or 100 µM overnight. HLA-E expression was analyzed by flow cytometry using APC-conjugated mouse anti-HLA-E antibody. ns, not significant; *p < 0.05; ***p<0.001.
