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. 2023 Jul 17;14:1217809. doi: 10.3389/fimmu.2023.1217809

Figure 4.

Figure 4

Inhibition of human NK cell degranulation by 5GKO cells expressing HLA-E and HLA-G molecules. rhIL-2 treated PBMCs (n=5) were co-cultured with 5GKO, 5GKO.HLA-E, 5GKO.HLA-G, and 5GKO.HLA-E.HLA-G cells for 2 hours. NK cell degranulation was accessed by CD107a surface expression. (A) Gating strategy to identify and refine NK cell population. (B) Representative flow plots showing NK cell degranulation in response to the simulation of the modified porcine cells by assessing the percentage of CD107a positive cells in CD3-CD56+ population. PBMC alone was used as a control. (C) Three independent experiments were performed to evaluate NK cell degranulation. Data presented as mean ± SEM. Ordinary one-way ANOVA multiple comparisons test was used to analyze the differences among multiple groups. 5GKO.HLA-E.HLA-G was selected as a control group. **p < 0.01; ***p < 0.001; ****p < 0.0001.