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. 2023 Jul 17;14:1217809. doi: 10.3389/fimmu.2023.1217809

Figure 5.

Figure 5

Human antibody reactivity to 5GKO cells expressing HLA-E and/or HLA-G molecules. 5GKO, 5GKO.HLA-E, 5GKO.HLA-G, and 5GKO.HLA-E.HLA-G cells were incubated with heat-inactivated human sera (n = 20), and then stained with goat anti-human IgG Alexa Fluor 488 or donkey anti-human IgM Alexa Fluor 647. Each cell line stained with the secondary antibody was used as a negative control. Human antibody binding was assessed by flow cytometry. Data presented as mean ± SEM. Ordinary one-way ANOVA multiple comparisons test was used to analyze the differences in (A) IgG binding with low PRA sera, (B) IgG binding with high PRA sera, (C) IgM binding with low PRA sera, and (D) IgM binding with high PRA sera, among multiple groups. (E) Comparison of low PRA sera and high PRA sera in IgG binding to individual modified pig cells was analyzed by the Student’s t-test. (F) Comparison of low PRA sera and high PRA sera in IgM binding to individual modified pig cells was analyzed via Student’s t-test. ns, not significant.