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. 2023 Jul 11;26(8):107333. doi: 10.1016/j.isci.2023.107333

Figure 3.

Figure 3

PAK1 inhibition by FRAX486 or IPA-3 alleviates BBB disruption during focal cerebral ischemia/reperfusion

(A) Representative fluorescence micrographs of brain cryosections from the mice subjected to MCAO/R for 3 h or 24 h. The TRITC-dextran (4.4 kDa) and FITC-dextran (70 kDa) leakage under different conditions (+vehicle, + FRAX486, + IPA-3). Scale bar = 5 mm.

(B and C) The quantitative analysis of the TRITC-dextran (4.4 kDa) and FITC-dextran (70 kDa) leakage volume. The TRITC-dextran (4.4 kDa) and FITC-dextran (70 kDa) leakage were lessened by FRAX486 (2 μmol/kg weight) or IPA-3 (10 μmol/kg weight).

(D) Representative image of microvessel leakage in striatum at 3 h reperfusion after MCAO. The vessels were filled with 70 kD FITC-dextran and the white arrow shows the leakage site on microvessels. Scale bar = 50 μm. Data are expressed as mean ± SEM (n = 3). ∗p < 0.05 ∗∗p < 0.01, ∗∗∗p < 0.001 versus vehicle group, one-way ANOVA with Dunnet’s post hoc test.