Skip to main content
. 2023 Jul 11;26(8):107333. doi: 10.1016/j.isci.2023.107333

Figure 6.

Figure 6

PAK1 contributes to the stress fiber formation in hCMEC/D3 and bEnd.3 cells during OGD/R

(A and B) Representative fluorescence image of F-actin staining in hCMEC/D3 and bEnd.3 cells with or without PAK1 inhibition (FRAX486, 1 μmol/L or IPA-3, 10 μmol/L). Scale bar = 20 μm.

(C and D) Statistic analysis showing reduced mean fluorescence intensity (MFI) of F-actin with PAK1 inhibitors or siRNA in hCMEC/D3 cells. Data are expressed as mean ± SEM (n = 4).

(E and F) Statistic analysis showing reduced MFI of F-actin with PAK1 inhibitors or siRNA in bEnd.3 cells. Data are expressed as mean ± SEM (n = 5). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 versus ctrl (C, E) or ctrl + siNC (D, F) group, #p < 0.05, ##p < 0.01, ###p < 0.001 versus OGD + vehicle (C, E) or OGD + siNC (D, F) group. All analyzed by one-way ANOVA with Sidak’s post hoc test.