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. 2023 Jul 11;26(8):107333. doi: 10.1016/j.isci.2023.107333

Figure 7.

Figure 7

Stress fiber formation on ischemic microvessels is decreased by the administration of PAK1 inhibitors

(A) Immunofluorescence images showing the colocalization of F-actin (green) with microvessel markers IB4 (red) in ipsilateral and contralateral hemisphere from control mice. Scale bar = 20 μm.

(B) The F-actin fluorescence of the ipsilateral and contralateral hemisphere in the same coronal sections from ischemic mice with or without PAK1 inhibition (FRAX486, 2 μmol/kg weight or IPA-3, 10 μmol/kg weight). To show a length of full vessel, the 7 μm thick z stack was acquired and processed maximum intensity projection. Scale bar = 20 μm.

(C) Quantitative analysis showing the much more obvious signal of F-actin MFI on region of interest (ROI) in ipsilateral hemisphere compared to contralateral hemisphere. FRAX486 (2 μmol/kg weight) or IPA-3 (10 μmol/kg weight) treatment reduced F-actin MFI on ipsilateral hemisphere. Data are expressed as mean ± SEM (n = 3). ∗∗∗p < 0.001 versus contralateral + vehicle; ##p < 0.01, ###p < 0.001 versus ipsilateral + vehicle, two-way ANOVA with Turkey’s post hoc test.