REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-PAK1	Cell Signaling Technology	Cat#2602; RRID: AB_330222
Rabbit polyclonal anti-p-PAK1 (Ser144)	Cell Signaling Technology	Cat#2606; RRID: AB_2299279
Mouse anti-GAPDH	Proteintech	Cat# 60004; RRID: AB_2107436
Rabbit monoclonal anti-PAK1	Abclonal	Cat#A19608; RRID: AB_2862697
Rabbit anti-ve-cadherin	Abclonal	Cat#A0734; RRID: AB_2757370
Rabbit anti-occludin	Invitrogen	Cat#71-1500; RRID: AB_2533977
Rabbit anti-ZO-1	Invitrogen	Cat#40-2200; RRID: AB_2533456
HRP conjugated goat anti-rabbit	Millipore	Cat# AP307P, RRID: AB_92641
HRP conjugated goat anti-mouse	Millipore	Cat# AP308P, RRID: AB_11215796
Alexa Fluor 488 conjugated goat anti-rabbit	Invitrogen	Cat#A32731; RRID: AB_2633280
Chemicals, peptides, and recombinant proteins
DyLight 594-conjugated lectin	Vector labs	Cat#DL-1177-1
DyLight 594-conjugated GSL-isolectin B4	Vector labs	Cat#DL1207
FITC-dextran, 70 kDa	Sigma-Aldrich	Cat#53471
TRITC-dextran, 4.4 kDa	Sigma-Aldrich	Cat#T1037
Fluorescein (FITC)-phalloidin	Sigma-Aldrich	Cat#P5282
2,3,5-triphenyltetrazolium hydrochloride (TTC)	Sigma-Aldrich	Cat#T8877
FRAX486	Selleck	Cat#S7807
IPA-3	Selleck	Cat#S7093
Critical commercial assays
IP lysis buffer	Beyotime	Cat#P0013
BCA protein assay kit	Beyotime	Cat#P0010
Protease inhibitor cocktails	Absin	Cat#ABS9161
Phosphatase inhibitor cocktails	Absin	Cat#ABS9162
Sulfo-NHS-SS-Biotin	Thermo Fisher Scientific	Cat#21331
Neutravidin agarose	Thermo Fisher Scientific	Cat#29202
Lipo8000	Beyotime	Cat#C0533
Cell counting kit 8 (CCK8)	APExBIO	Cat#K1018
Experimental models: Cell lines
bEnd.3	ATCC	N/A
hCMEC/D3	ATCC	N/A
Experimental models: Organisms/strains
Mouse: wild type: C57BL/6J	Xuzhou Medical University	N/A
Oligonucleotides
siRNA targeting PAK1	Synthetized by GenePharma	5’-GCAUCAAUUCC UGAAGAUUTT-3’
Negative control of siRNA	Synthetized by GenePharma	5’-UUCUCCGAACG UGUCACGUTT-3’
Software and algorithms
Prism 7.0	Graphpad	http://www.graphpad.com
ImageJ	NIH	https://imagej.nih.gov/ij/