Table 2.
List of primary and secondary antibodies used for protein detection by Western blot.
Antibodies | Dilution | Supplier | Host | Molecular weight (kDa) | Specific target species | Validation for avian species |
---|---|---|---|---|---|---|
Chemerin | 1/1000 | Agrobio | Mouse | 18 | Chicken | (Estienne et al., 2020b) |
Adiponectin | 1/1000 | Agrobio | Rabbit | 25–28 | Bovine | (Diot et al., 2015b) |
Visfatin | 1/1000 | Agrobio | Rabbit | 52 | Chicken | (Diot et al., 2015b, 2015a) |
Adipolin | 1/1000 | Novus Biological | Rabbit | 25–32 | Human | |
Rabbit | 1/50000 | Biorad | Goat | |||
Mouse | 1/50000 | Interchim | Goat |