REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-NOSIP	Proteintech	Cat# 27979-1-AP; RRID: AB_2881027
Anti-Tubulin	CST	Cat#5335;
HRP- conjugated β-actin	Proteintech	Cat#HRP-66009; RRID:AB_2883836
Chemicals, peptides, and recombinant proteins
RPMI-1640 medium	Gibco	Cat# 11875119
McCoys 5A medium	Gibco	Cat# 16600082
DMEM medium	Gibco	Cat# 11965092
Quercetin	Sigma	Cat# PHR1488
Experimental models: Cell Lines
Hep3B	Shanghai Cell Bank	Cat# SCSP-5045
L-02	Shanghai Cell Bank	Cat# BFN608006124
SNU-449	Shanghai Cell Bank	Cat# BFN60800681
SMCC-7721	Shanghai Cell Bank	Cat# BFN60800687
Critical commercial assays
NOSIP siRNA	Gene Pharma	NA
PrimeScript cDNA synthesis kit	vazyme	Cat# R323-01
QIAquick PCR purification Kit	vazyme	Cat# Q711-02
NOSIP plasmid	Youbio	NA
Lipofectamine 3000	ThermoFisher	Cat# L3000008
Lipofectamine 2000	ThermoFisher	Cat# 11668019
DAF-2 DA	上海翌圣	CAS NO.:205391-02-2
1400W Dihydrochloride	MCE	Cat# HY-18731; Lot No.28897
Carboxy-PTIO potassium	MCE	Cat#HY-18734; Lot No.157072
Software and algorithms
GraphPad Prism	GraphPad	Version 8.0
Flowjo	BD Biosciences	Version 10
Image J	NIH	https://imagej.nih.gov/ij/
Others
Transcriptomic data of HCC	TCGA	https://portal.gdc.cancer.gov/