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FTO reduced EGR2 methylation and promoted EGR2 expression. (A) qRT-PCR validation results for 5 candidate genes. (B) The relative expression of EGR2 was detected by WB in FTO-overexpressed Du145 and PC-3 cells. (C) Methylation levels of EGR2 were determined using MeRIP-PCR in Du145 cells after FTO overexpression. * p < 0.05, ** p < 0.01, *** p < 0.0001.
