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. 2023 Jun 26;299(8):104968. doi: 10.1016/j.jbc.2023.104968

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© 2023 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

The effect of phospho-mutations on the conformations of Ykt6ΔC.A, FRET efficiency distribution profiles of rYkt6ΔC (gray) and rYkt6ΔC-S174D (brown) systems. B, comparison of the distribution ratios of conformational states of rYkt6ΔC (gray) and rYkt6ΔC-S174D (brown). C, ITC measurement of the interaction between DPC and rYkt6ΔC-S174D. D, FRET efficiency distribution profiles of yYkt6ΔC (blue) and yYkt6ΔC-S176D (olive drab). E, comparison of the population ratios of the conformational states of yYkt6ΔC (blue) and yYkt6ΔC-S176D (olive drab). F, FRET efficiency distribution profiles of WT rYkt6ΔC (dim gray) and yYkt6ΔC-S176D (olive drab). Significance was determined by the two-tailed t test. ∗p < 0.05, ∗∗p < 0.01. DPC, dodecylphosphocholine; ITC, isothermal titration calorimetry.