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. 2023 Jul 17;15:1169211. doi: 10.3389/fnagi.2023.1169211

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Copyright © 2023 Chen, Tzekov, Su, Zhu, Han and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PRDX6 overexpression attenuates H₂O₂-induced oxidative damage in ARPE-19 cells. ARPE-19 cells were infected with lentivirus containing a control plasmid or the pLV-Flag PRDX6 plasmid for 48 h and were then treated with H₂O₂. (A) Cell viability was measured using an MTT assay. (B) Cell death analysis by flow cytometry. (C) Quantification of ARPE-19 cell death, n = 3. (D) Flow cytometric analysis of ROS levels, with DCFDA staining. (E) Histograms representing the relative ROS levels, which were fluorescence densities of DCF transformed from DCFDA, n = 3, *P < 0.05, **P < 0.01.