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. Author manuscript; available in PMC: 2023 Jul 31.
Published in final edited form as: J Vis Exp. 2022 Aug 9;(186):10.3791/63324. doi: 10.3791/63324

Figure 2: Mounting and imaging Zebrafish embryos.

Figure 2:

(A) The concave well imaging slide with zebrafish embryo under the microscope. (B) Magnified view of the well. Fill the wells ¾ of volume with E3 medium. (C) Positioning of the embryo should be on its left side. (D) Ventricle can be seen clearly in this configuration (zoomed image on left, ventricle borders are highlighted). Anterior is to the left. (E) Moving red blood cells (RBC) in major vessels, such as the dorsal aorta or the Posterior Cardinal Vein. Anterior is to the left.