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. Author manuscript; available in PMC: 2023 Jul 31.
Published in final edited form as: J Vis Exp. 2022 Aug 9;(186):10.3791/63324. doi: 10.3791/63324

Figure 8: Assessment of cardiac function.

Figure 8:

(A) Dorsal aorta blood flow analysis, (B) Posterior Cardinal Vein (PCV) blood flow analysis. 1–4 cell stage zebrafish embryos were injected as groups with scrambled MO, Hand2 MO, Hand2 MO + Hand2 mRNA rescue, and scrambled MO + Hand2 mRNA rescue. Un-injected embryos were used as control. All data are presented as mean ± SEM (6 embryos were used in each group; experiment was performed in triplicate). The analysis was done by one-way ANOVA with Sidak post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.