(A, C) FPLC fractions from WT, MCU−/−, and MICU1−/− HEK293T cells were subjected to Western blotting for MIC60 (A), and CHCHD2 (C). Western blots are representative of 4 independent experiments. (B, D) Densitometry was performed to quantify MICOS component distribution in different MW fractions in Fig. 4A and 4B. Error bar= SEM. ****, p<0.0001; **, p<0.01. n= 4 independent experiments.