TABLE 1.
Distribution of HHV-6 sequences in cell fractions sorted from the peripheral blood of subjects with high-copy-number latent viral infections
Patient | HHV-6 DNA in sorted cell fractionsa
|
||||
---|---|---|---|---|---|
CD16+ granulocytes | CD14+ monocytes | CD19+ B lymphocytes | CD4+ T lymphocytes | CD8+ T lymphocytes | |
1 | 10 | 10 | 102 | 102 | 103 |
2 | 102 | 102 | 103 | 103 | 104 |
3 | 10 | 10 | 102 | 102 | 103 |
Monocyte and lymphocyte populations were >98% pure, while sorted CD16+ granulocytes were >99% pure. Tenfold dilutions of 105 FACS-purified cells (105, 104, 103, 102, and 10 cells) were tested for HHV-6 DNA by PCR, and the minimal numbers of positive cells are given.