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. 2023 Jul 17;14:1164534. doi: 10.3389/fpls.2023.1164534

Figure 1.

Figure 1

Sequence analysis, transcription activation activity, DNA binding activity, and gene expression of CdWRKY2. (A) The protein structure of CdWRKY2 with two WRKY domains. (B) Phylogenetic tree constructed with CdWRKY2 and other WRKY proteins in Arabidopsis by the MEGA 5.0 program with neighbor-joining method. (C) Transcriptional activation analysis of CdWRKY2 in yeast cells. The negative control vector was pGBKT7. (D) W-box and mutated W-box (mW-box) was constructed into pAbAi vector, respectively, and were used to check DNA binding activity of CdWRKY2 using the yeast one-hybrid method. RT-qPCR analysis of CdWRKY2 expression in the shoots and roots under salt condition (200 mM NaCl for 0, 6, and 12 h) (E). The internal control gene used for salt treatment was CdPP2A. Data are represented as means ± SD of three independent replicates, and different letters indicate significant differences at p < 0.05 by two -way analysis of variance (ANOVA tests) with Tukey’s post‐hoc test.