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. 2023 Jul 17;14:1164534. doi: 10.3389/fpls.2023.1164534

Figure 4.

Figure 4

The phenotype of the CdWRKY2 transgenic Arabidopsis lines. The seeds of wild type (Col-0) and CdWRKY2 overexpression lines (AtOE10-4 and AtOE6-1) were germinated and grown for 4 days on 1/2 MS. The seedlings were transferred to 1/2 MS containing 0 mM NaCl (CK), 100 mM NaCl (Salt), or 100 mM NaCl with 0.1 μM IAA (Salt+IAA), respectively. The morphological parameters were measured after being grown vertically for 7 days. (A) Images of overexpression line (AtOE10-4) and wild type (Col-0) grown 7 days on CK and salt conditions. Bar = 1 cm. (B) Relative expression of CdWRKY2 in AtOE10-4 and AtOE6-1. (C) Images of AtOE10-4 and Col-0 grown 7 days on 1/2 MS containing salt and salt plus 0.1 μM IAA, respectively. Bar = 1 cm. (D) Shoot fresh weight of overexpression and wild-type plants grown under CK and salt conditions. (E) Visible LR number of overexpression and wild-type plants grown under CK and salt conditions. Shoot fresh weight (F) and visible LR number (G) of overexpression and wild-type plants grown under salt and salt plus IAA conditions. Data are represented as means ± SD of at least 15 seedlings from three independent treatments. Two-way ANOVA test was used and the data were further compared by Tukey’s post‐hoc test. Different letters displayed represent significant differences at p < 0.05 statistically.