Figure 5.

GUS staining and auxin pathway-related gene expression of Col-0 and the CdWRKY2 overexpression background. The seeds of wild type (Col-0), CdWRKY2-overexpressing line (AtOE10-4), plants expressing DR5::GUS in Col-0 and AtOE10-4 background were germinated for 4 days. The seedlings were transferred to 1/2 MS that contained either 0 mM NaCl (CK) or 100 mM NaCl (Salt) for 7 days. GUS staining in LR primordia (LRP) (A), emerged LR tip (B), and visible LR (C). Bar = 1000 μm. Gene expression of YUC1 (D), YUC4 (E), PIN1 (F), and UGT84B2 (G) in Col-0 and AtOE10-4 roots under CK and salt conditions were determined by RT-qPCR using UBQ10 as an internal control reference gene. Transcript levels of each sample were normalized to the expression of untreated wild-type plants. Three independent replicates were performed. Two-way ANOVA test was used and the data were further compared by Tukey’s post‐hoc test. Different letters represent significant differences at p < 0.05.