Table 3.
Overview of donor sample properties and inhibition zones (halos) in the in vitro assay for donor selection.
| Donor | L. crispatus % | L. gasseri % | L. iners % | L. jensenii % | Other lactobacilli % | Selected vaginal dysbiosis-associated bacteria % | pH | Viable cells or CFU/dose | Halo (mm) un-diluted | Halo (mm) 5× diluted |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 88.7 | <0.05 | 4.0 | 7.2 | <0.05 | 0 | 4.1 | 3.1E08 | 3 | 1.5–2 |
| 2 | 97.6 | 0 | 0.3 | 2.0 | 0.1 | 0 | 3.8 | 9.9E08 | 3 | 0 |
| 3 | 97.3 | 2.2 | 0 | 0.1 | 0.2 | 0 | 4.0 | 3.4E08 | 0 | 0 |
| 4 | 0 | 0 | 99.6 | 0 | 0 | 0.1 | 3.9 | 1.0E11 | 0 | 0 |
The percentages for each species are relative abundances as determined by whole metagenome shotgun sequencing of the donor sample used in the assay. The pH and CFU are the pH and CFU of the donor sample used in the assay. The viable cells or CFU was measured in the fresh sample; see Table 2 for CFU in the stored sample of the selected donor sample.