TABLE 1.
In vivo spontaneous reactivationa
| Virus or P value comparison | No. of virus-positive rabbits/total no. of rabbits (%) or P value | No. of virus-positive eyes/total no. of eyes (%) or P value | No. of virus-positive eye swabs/total no. of eye swabsb (%) or P value |
|---|---|---|---|
| Viruses | |||
| 17syn+ | 6/8 (75) | 11/15 (73) | 35/297 (11.8) |
| 17ΔSty | 3/13 (23) | 4/25 (16) | 11/492 (2.2) |
| 17ΔSty-Res | 7/10 (70) | 13/19 (64) | 38/377 (10.1) |
| P value comparisonc | |||
| 17ΔSty vs 17syn+ | 0.03 | 0.0005 | <0.0001 |
| 17ΔSty vs 17ΔSty-Res | 0.04 | 0.0006 | <0.0001 |
| 17syn+ vs 17ΔSty-Res | 1.0 | 1.0 | 0.53 |
| Virusesd | |||
| McKrae | 25/30 (83) | 44/60 (73) | 183/1,570 (11.7) |
| dLAT371 | 5/6 (83) | 9/12 (75) | 38/290 (13.1) |
| dLAT2903 | 13/28 (46) | 15/56 (27) | 37/1,516 (2.4) |
| P value comparisonc | |||
| 17syn+ vs McKrae | 0.62 | 1.0 | 0.42 |
| 17ΔSty vs dLAT371 | 0.04 | 0.0008 | <0.0001 |
| 17ΔSty vs dLAT2903 | 0.19 | 0.40 | 0.93 |
a
Rabbits were infected with 2 × 105 PFU of the indicated virus per eye. Tears (eye swabs) were collected daily from latently infected rabbits from day 20 to 39 p.i. Spontaneous reactivation was monitored by culturing tears for the presence of infectious HSV-1 as described previously (7).
b
The total numbers of cultures are slightly less than the numbers of eyes times 20 days, because occasional cultures were lost due to contamination.
c
Two-sided Fisher exact test or chi-square test if the numbers were too large for the Fisher exact test. Analyses were done with the personal computer program Instat. A P of <0.05 was considered significant.
d
Data reproduced from a previous publication (19).